Serial m1b altervista org

With this method, it achieves both robustness and speed. For example, a 2Kx2K, 1 degree tilt series can be acquired in about minutes with a four-port readout CCD camera. The method and the program are described in this paper: Mastronarde, D. Automated electron microscope tomography using robust prediction of specimen movements. The prediction method was first described in this abstract from the MSA meeting, Mastronarde, D.

SerialEM: A program for automated tilt series acquisition on Tecnai microscopes using prediction of specimen position. Microscopy and Microanalysis Vol. SerialEM is also widely used to acquire data for single particle reconstruction.

There is a routine that finds hole positions automatically and one that can combine them into an optimal subset of positions for acquiring from multiple holes.

The basic framework for this automated acquisition is through the Navigator module, but there is currently no user interface for the various additional options needed for efficient, high-quality data acquisition. Such options are provided by SerialEM's scripting capability, and there are various sophisticated collections of scripts available in the EM community. In addition to automating data acquisition, the program provides an integrated user interface for image acquisition, display, and storage.

It looks like this: Click image for full-sized view. Other notable features include: The program provides a flexible interface for user intervention. When acquiring a tilt series, the user can stop if necessary, adjust position or focus, and even conveniently back up to a previous tilt angle and replace the stored data from that point onward.

Other kinds of acquisition also provide an ability to stop and restart. There is a low dose mode, in which focusing and tracking are done in a separate area to minimize exposure of the area being recorded. The program allows one to set up the magnification and illumination independently for up to 4 different areas, where these settings are invoked simply by taking a picture of the particular area.

SerialEM can automatically acquire a montage of overlapping frames, and it can acquire a tilt series of such montaged images. The images are written to a single file that can be viewed immediately in 3dmod. It implements several features to make the GIF more convenient to use, such as automatic switching between regular magnifications with the viewing screen down and the EFTEM lens series needed to use the GIF with the screen up. Both low dose mode and montaging can be used with the filtering.

The program supports imaging and tilt series acquisition in STEM mode. There is a Navigator window that allows one to store and return to specimen positions, make low-magnification maps, mark positions on maps, return to marked positions reliably, and automatically acquire images, tilt series, or montages at a series of positions.

The program has a scripting capability that includes variables, 1D and 2D arrays, simple arithmetic statements, looping, and conditional statements. It is most commonly used to acquiring images automatically for single-particle reconstruction; some scripts are available for this purpose. The script capability also provides a relatively easy way to add commands requested by users for specialized or experimental use.

In addition, you can download and view the compiled help file for the development version after unzipping on any Windows machine. The on-line help and compiled file for the development version are continually updated and will often show features that are not in the current release version.

Since , it has been maintained and developed with funding that comes from users, which has allowed us to focus on coding and documenting instead of grant-writing.

Since , funds have also been used to pay Guenter Resch to provide user support. You can help make SerialEM a community-supported resource by having your laboratory or microscope facility contribute to the further development and continued availability of the program.

The mechanism for doing this is to purchase priority annual support, so that the expense represents a tangible item that can be paid from grant or institutional funds. In September , we transitioned to having these payments made to Guenter Resch's company, Nexperion.

In addition to directly funding Guenter's assistance to users, the majority of the funds are used to compensate David Mastronarde for his work on SerialEM. We offer three options, allowing you to pick the size of your payment based on your financial resources and the importance of SerialEM to your work. The options are:. What happens if you do not pay? Although the first option represents the kind of support that we provide routinely, payment for this support is voluntary.

We do answer questions and deal with problems from non-paying users, but may take longer to do so. Why is it important to contribute? User contributions are important both financially and because they are not tied to specific developments and represent the general user interest. What does the change in mean for the future of SerialEM? This method must be called before attempting to use the HardwareSerial object typically, you call this in your setup function.

Disables the USART associated with this object, allowing any associated communication pins to be used for other purposes. Returns the next available, unread character. If there are no available characters you can check this with available , the call will block until one becomes available. That is, clears any buffered characters, so that the next character read is guaranteed to be new. Negative values will be prefixed with a '-' character.

The base value 2 corresponds to binary, 8 to octal, 10 to decimal, and 16 to hexadecimal. Print n , accurate to 2 digits after the decimal point. Edit I have found error the error is here: client. Use a POST request. The data goes after a blank line after all the headers. But why? Is the data really that private? The form has a submit button, if it is to do anything with the input text field data.

In general, that submit button sends another GET request to the same server that generated the form. No, the Arduino can not click a button.